Bamfield Community Huu-ay-aht
Abalone Project
| broodstock | DNA analysis | hatchery
| settlement | outplanting |
- Outplanting - Spring 2005
In the spring of 2005, the BHCAP volunteer
divers outplanted about 75,000 juvenile abalone in Barkley Sound. This
time, the technique used was a bit different - Juvenile abalone were
settled onto pieces of concrete blocks, inserted into black plastic
crates in the hatchery, then delivered into the study site by divers.
These "Abitats" were
then surrounded by rocks. Here we have some photos and a video clip
to show how it all went.
In the first pinto abalone juvenile out-planting conducted by BHCAP, 1 year old, 8mm average shell length animals were released. Since then, juvenile out-planting has focused on the release of much younger and smaller animals, such that the juveniles that were released in early 2005 were approximately 1mm in length and 1 month old. The out-planting technique used for these tiny juveniles is also different from the larger juveniles. The tiny animals are fragile and must be handled on their settlement substrate, not removed from the substrate. Thus, out-planting modules were developed on which the larvae were set in the hatchery, incubated for 1 month and then transported to the field for out-planting.
There are several reasons for out-planting (larvae and) young/tiny juveniles. Animals that have spent less time in the hatchery are less likely to be habituated to hatchery conditions and more able to easily adapt to the field environment. Food sources of young juveniles are the same in the hatchery as in the field (a natural assemblage of diatoms) and due to the use of this "field-type" food source (i.e. before conversion to a largely kelp diet), shell colour of juveniles is the same as in the field. Thus released animals are cryptically coloured and less likely to be identified by visual predators. Innate behavioural responses such as hiding during the daytime and the escape response to certain predators are present in young juveniles and most likely to be preserved if juveniles are released to the field at an early age. And not least of all reasons are the practical considerations of the greater ease and lower cost of production of (larvae and) young juveniles by a hatchery. To accomplish the long-term goal of widespread population rebuilding of pinto abalone by the out-planting of hatchery stock, it is important that the economics of animal production are realistic. Thus BHCAP has chosen larvae and tiny juveniles for their out-planting.
The same larval rearing process was used for both larval and juvenile out-planting. Instead of taking competent larvae into the field, and setting them immediately prior to release, larvae for juvenile production were set in the hatchery onto out-planting modules. Later the modules were transferred to the field. The usual BHCAP hatchery practice is to settle competent larvae onto PVC wavy plates, held in baskets and reared in raceway tanks. However, for out-planting, this procedure is altered.
BHCAP
used broken concrete blocks as the substrate for settlement.
There is anecdotal evidence to suggest that competent pinto abalone
larvae have an affinity for concrete. Concrete and coralline algae
both have calcium carbonate (in the cell walls of the algae) in common.
For three months prior to settlement, the concrete blocks were conditioned
in running seawater, to leach out any undesirable substances present
in new concrete.
During the conditioning period, the blocks were colonized by a natural
assemblage of diatoms that came from the running seawater.
Out-planting modules were constructed to contain the concrete blocks.
The BHCAP modules were relatively small (in comparison with the abalone
aggregation modules utilized by some B.C. abalone stewardship groups)
in order to be easily and carefully handled by boat crew and divers.
The fragile nature of the tiny juvenile abalone that the modules housed
necessitated handling by hand rather than mechanically. The modules used
were plastic crates, with plastic mesh fastened to cover the bottom,
top and side openings in order to exclude larger predators. While it
might be desirable to exclude all predators until the abalone juveniles
reached a larger size, it was also necessary to allow the abalone to
leave the module when necessary, in addition to leaving adequate openings
for water circulation through the module. The concrete block pieces were
stacked randomly inside the crate leaving spaces of various sizes between
the blocks. While the modules were in the hatchery, the mesh tops were
left off and were attached immediately prior to out-planting.
The modules, including the conditioned blocks were placed into a hatchery
raceway tank and the blocks were rotate at weekly intervals to allow
the diatom film to continue to grow over all surfaces of the blocks.
Supplemental diatoms were also added to accelerate coverage.

A good coverage of diatoms was established and prior
to settlement, the blocks were lightly washed to remove loosely attached
diatoms, which would interfere with firm attachment of the settling larvae.
At settlement, the water level in the tanks was reduced to be just deep
enough to completely cover the concrete blocks. This was to ensure that
when the induced larvae were poured into the modules they were more likely
to remain in the modules rather than drifting out into the raceway tank.
The competent abalone larvae were treated with GABA in the hatchery lab
in a similar manner to that used on board the dive boat for larval out-planting.
The GABA was prepared.
The larvae were moved from the larval rearing chambers
where they had been raised for the previous 2 weeks, counted and immersed
into the GABA for treatment, then rinsed.

The larvae were removed from the filter and poured onto
the modules.


Three days after
settlement the water was check microscopically for any larvae that still
remain swimming and none were found. This indicated that settlement was
completed. A small sample was taken from the surface of some blocks and
living, crawling post-larvae were found. In this photograph the shell
is above the focal plane and the 2 cephalic tentacles at the head end
as well as the trailing edge of the foot can be seen in this crawling,
tiny juvenile.

After
the juveniles had spent one month on the concrete blocks in the hatchery,
they had reach approximately 600 µm shell
length and had developed epipodial tentacles as well as undergone new
shell growth.
The modules were out-planted at Scott's Bay on 31 March
2005. First, mesh lids were added to the top of the modules to prevent
predator entry. The modules were then placed into totes containing seawater
for transport on the MV Barkley Star dive boat. The divers carried the
modules in the totes to the sea floor to minimize water motion against
the blocks.


Video of modules with young abalone being outplanted by divers!
The modules were removed from the totes and wedged between
rocks to keep them in place.
The out-planting was done on a day with significant surf, which was beneficial
in allowing the divers to get a feeling for how much protection was required
to keep the modules in place.

This
out-planting was done at the end of March 2005. It is anticipated that
in 3-4 months time we will be able to revisit the modules and remove
some blocks to see if the juveniles are still present. Population surveys
done in future years will show whether the population of the area was
enhanced.
more outplanting events:
November 2003 | Summer 2004
| March 2005
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